TY - JOUR

T1 - Validation of a radioimmunoassay for the determination of total corticosterone in rat plasma

AU - Hjort Pedersen, Tine Karen

AU - Hansen, Åse Marie

AU - Lund, Søren Peter

AU - Garde, Anne Helene

PY - 2000/5/18

Y1 - 2000/5/18

N2 - A radioimmunoassay (RIA) for total corticosterone (CORT) in rat plasma requiring a plasma volume of 2μl was established. The importance of inactivating plasma corticosteroid-binding globulin (CBG), denatured by heat, before measuring CORT was shown. The method was evaluated and shown to be within analytical and statistical controls, with a recovery not significantly different from 100%. The limit of detection (LD) was 0.2μgdl-1. Intra-assay coefficients of variation (CVs) were 7.2% for plasma samples at 33.5μgdl-1 CORT (n=10) and 8.5% at 0.75μgdl-1 CORT (n=18). Inter-assay CVs were 6.8% at 23.1μgdl-1 CORT (n=8) and 5.4% at 8.9μgdl-1 CORT (n=8). Validation of the method was performed using a group of male rats (Mol:WIST Han; M and B A/S, Denmark; n=12), housed under conventional environmental conditions from the age of 4 weeks. The rats were 3 and 7/2 months old when they underwent the blood sampling procedure. The result showed that the method had a capacity to detect CORT concentrations comparable with previous reported basal concentrations. Finally, the possible stress inducing effect of the blood sampling procedure was examined using two groups of male rats housed under either conventional or enriched environmental conditions. The result indicated that conventional environment housing induces slightly stressed animals compared to enriched housing. Enriched housing may provide an environment that makes it possible for rats to compensate for a stressful situation, i.e., the blood sampling procedure. Copyright (C) 2000 Elsevier Science B.V.

AB - A radioimmunoassay (RIA) for total corticosterone (CORT) in rat plasma requiring a plasma volume of 2μl was established. The importance of inactivating plasma corticosteroid-binding globulin (CBG), denatured by heat, before measuring CORT was shown. The method was evaluated and shown to be within analytical and statistical controls, with a recovery not significantly different from 100%. The limit of detection (LD) was 0.2μgdl-1. Intra-assay coefficients of variation (CVs) were 7.2% for plasma samples at 33.5μgdl-1 CORT (n=10) and 8.5% at 0.75μgdl-1 CORT (n=18). Inter-assay CVs were 6.8% at 23.1μgdl-1 CORT (n=8) and 5.4% at 8.9μgdl-1 CORT (n=8). Validation of the method was performed using a group of male rats (Mol:WIST Han; M and B A/S, Denmark; n=12), housed under conventional environmental conditions from the age of 4 weeks. The rats were 3 and 7/2 months old when they underwent the blood sampling procedure. The result showed that the method had a capacity to detect CORT concentrations comparable with previous reported basal concentrations. Finally, the possible stress inducing effect of the blood sampling procedure was examined using two groups of male rats housed under either conventional or enriched environmental conditions. The result indicated that conventional environment housing induces slightly stressed animals compared to enriched housing. Enriched housing may provide an environment that makes it possible for rats to compensate for a stressful situation, i.e., the blood sampling procedure. Copyright (C) 2000 Elsevier Science B.V.

KW - Corticosterone

KW - Radioimmunoassay

KW - Rats

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=0034682228&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(00)00812-6

DO - 10.1016/S0003-2670(00)00812-6

M3 - Journal article

AN - SCOPUS:0034682228

VL - 413

SP - 63

EP - 69

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

SN - 0924-2031

IS - 1-2

ER -