Lung inflammation and genotoxicity in mice lungs after pulmonary exposure to candle light combustion particles
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Lung inflammation and genotoxicity in mice lungs after pulmonary exposure to candle light combustion particles. / Skovmand, Astrid; Damiao Gouveia, Ana Cecilia; Koponen, Ismo Kalevi; Møller, Peter; Loft, Steffen; Roursgaard, Martin.
I: Toxicology Letters, Bind 276, 05.07.2017, s. 31-38.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Lung inflammation and genotoxicity in mice lungs after pulmonary exposure to candle light combustion particles
AU - Skovmand, Astrid
AU - Damiao Gouveia, Ana Cecilia
AU - Koponen, Ismo Kalevi
AU - Møller, Peter
AU - Loft, Steffen
AU - Roursgaard, Martin
N1 - Copyright © 2017 Elsevier B.V. All rights reserved.
PY - 2017/7/5
Y1 - 2017/7/5
N2 - Candle burning produces a large amount of particles that contribute substantially to the exposure to indoor particulate matter. The exposures to various types of combustion particles, such as diesel exhaust particles, have been associated with increased risk of lung cancer by mechanisms that involve oxidative stress, inflammation and genotoxicity. The aim of this study was to compare pulmonary effects of candle light combustion particles (CP) with two benchmark diesel exhaust particles (A-DEP and SRM2975). Intratracheal (i.t.) instillation of CP (5mg/kg bodyweight) in C57BL/6n mice produced a significant influx of alveolar macrophages and polymorphonuclear leukocytes and increased concentrations of proteins and lactate dehydrogenase activity in bronchoalveolar fluid. Lower levels of these markers of inflammation and cytotoxicity were observed after i.t. instillation of the same dose of A-DEP or SRM2975. The i.t. instillation of CP did not generate oxidative damage to DNA in lung tissue, measured as DNA strand breaks and human 8-oxoguanine glycosylase-sensitive sites by the comet assay. The lack of genotoxic response was confirmed in lung epithelial (A549) cells, although the exposure to CP increased intracellular levels of reactive oxygen species. In conclusion, pulmonary exposure to particles from burning candles is associated with inflammation and cytotoxicity in the lungs.
AB - Candle burning produces a large amount of particles that contribute substantially to the exposure to indoor particulate matter. The exposures to various types of combustion particles, such as diesel exhaust particles, have been associated with increased risk of lung cancer by mechanisms that involve oxidative stress, inflammation and genotoxicity. The aim of this study was to compare pulmonary effects of candle light combustion particles (CP) with two benchmark diesel exhaust particles (A-DEP and SRM2975). Intratracheal (i.t.) instillation of CP (5mg/kg bodyweight) in C57BL/6n mice produced a significant influx of alveolar macrophages and polymorphonuclear leukocytes and increased concentrations of proteins and lactate dehydrogenase activity in bronchoalveolar fluid. Lower levels of these markers of inflammation and cytotoxicity were observed after i.t. instillation of the same dose of A-DEP or SRM2975. The i.t. instillation of CP did not generate oxidative damage to DNA in lung tissue, measured as DNA strand breaks and human 8-oxoguanine glycosylase-sensitive sites by the comet assay. The lack of genotoxic response was confirmed in lung epithelial (A549) cells, although the exposure to CP increased intracellular levels of reactive oxygen species. In conclusion, pulmonary exposure to particles from burning candles is associated with inflammation and cytotoxicity in the lungs.
KW - Journal Article
U2 - 10.1016/j.toxlet.2017.04.015
DO - 10.1016/j.toxlet.2017.04.015
M3 - Journal article
C2 - 28465192
VL - 276
SP - 31
EP - 38
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
ER -
ID: 179121830