TY - JOUR

T1 - Elevated lipid class concentrations in females with anorexia nervosa before and after intensive weight restoration treatment—A lipidomics study

AU - Hussain, Alia Arif

AU - Bilgin, Mesut

AU - Carlsson, Jessica

AU - Foged, Mads Møller

AU - Mortensen, Erik Lykke

AU - Bulik, Cynthia M.

AU - Støving, René Klinkby

AU - Sjögren, Jan Magnus

N1 - Publisher Copyright: © 2023 The Authors. International Journal of Eating Disorders published by Wiley Periodicals LLC.

PY - 2023

Y1 - 2023

N2 - Objective: To study the plasma lipidome of patients with anorexia nervosa (AN) before and after weight restoration treatment and report associations with AN subtypes and oral contraceptive pill (OCP) usage. Methods: Quantitative shotgun lipidomics analysis was used to study plasma lipids of 50 female patients with AN before and after weight restoration treatment and 50 healthy female controls (HC). The AN group was assessed with blood samples and questionnaires before and after weight restoration. Results: In total we quantified 260 lipid species representing 26 lipid classes of which 13 lipid class concentrations were elevated in patients with AN at admission compared with HC. Lipid classes remained elevated after weight restoration treatment of 84 days (median; interquartile range 28), and only the concentration of the ceramide lipid class increased between pre- and post-treatment (p =.03), whereas lysophosphatidylcholine (LPC, p =.02), ether-linked Phosphatidylcholine (LPCO, p =.02), and lysophosphatidylethanolamine (LPE, p =.009) decreased. Conclusion: In AN, 13 out of 26 lipid class concentrations were elevated at admission and remained elevated post-treatment. Ceramides increased further between pre- and post-weight restoration treatment, which could be related to the rapid weight gain during re-nutrition. Further research is needed to elucidate the effects of weight restoration treatment on short- and long-term lipid profiles in individuals with AN. Public Significance Statement: Lipidomics research can increase the understanding of AN, a complex and potentially life-threatening eating disorder. By analyzing lipids, or fats, in the body, we can identify biological markers that may inform diagnosis and develop more effective treatments. This research can also shed light on the underlying mechanisms of the disorder, leading to a better understanding of the processes involved in eating behavior.

AB - Objective: To study the plasma lipidome of patients with anorexia nervosa (AN) before and after weight restoration treatment and report associations with AN subtypes and oral contraceptive pill (OCP) usage. Methods: Quantitative shotgun lipidomics analysis was used to study plasma lipids of 50 female patients with AN before and after weight restoration treatment and 50 healthy female controls (HC). The AN group was assessed with blood samples and questionnaires before and after weight restoration. Results: In total we quantified 260 lipid species representing 26 lipid classes of which 13 lipid class concentrations were elevated in patients with AN at admission compared with HC. Lipid classes remained elevated after weight restoration treatment of 84 days (median; interquartile range 28), and only the concentration of the ceramide lipid class increased between pre- and post-treatment (p =.03), whereas lysophosphatidylcholine (LPC, p =.02), ether-linked Phosphatidylcholine (LPCO, p =.02), and lysophosphatidylethanolamine (LPE, p =.009) decreased. Conclusion: In AN, 13 out of 26 lipid class concentrations were elevated at admission and remained elevated post-treatment. Ceramides increased further between pre- and post-weight restoration treatment, which could be related to the rapid weight gain during re-nutrition. Further research is needed to elucidate the effects of weight restoration treatment on short- and long-term lipid profiles in individuals with AN. Public Significance Statement: Lipidomics research can increase the understanding of AN, a complex and potentially life-threatening eating disorder. By analyzing lipids, or fats, in the body, we can identify biological markers that may inform diagnosis and develop more effective treatments. This research can also shed light on the underlying mechanisms of the disorder, leading to a better understanding of the processes involved in eating behavior.

KW - anorexia nervosa

KW - ceramides

KW - contraceptive

KW - eating disorders

KW - lipids

KW - shotgun lipidomics

U2 - 10.1002/eat.24063

DO - 10.1002/eat.24063

M3 - Journal article

C2 - 37715358

AN - SCOPUS:85171299018

VL - 56

SP - 2260

EP - 2272

JO - International Journal of Eating Disorders

JF - International Journal of Eating Disorders

SN - 0276-3478

IS - 12

ER -