TY - JOUR

T1 - Single-Walled vs. Multi-Walled Carbon Nanotubes

T2 - Influence of Physico-Chemical Properties on Toxicogenomics Responses in Mouse Lungs

AU - Solorio-Rodriguez, Silvia Aidee

AU - Williams, Andrew

AU - Poulsen, Sarah Søs

AU - Knudsen, Kristina Bram

AU - Jensen, Keld Alstrup

AU - Clausen, Per Axel

AU - Danielsen, Pernille Høgh

AU - Wallin, Håkan

AU - Vogel, Ulla

AU - Halappanavar, Sabina

N1 - Publisher Copyright: © 2023 by the authors.

PY - 2023

Y1 - 2023

N2 - Single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) are nanomaterials with one or multiple layers of carbon sheets. While it is suggested that various properties influence their toxicity, the specific mechanisms are not completely known. This study was aimed to determine if single or multi-walled structures and surface functionalization influence pulmonary toxicity and to identify the underlying mechanisms of toxicity. Female C57BL/6J BomTac mice were exposed to a single dose of 6, 18, or 54 μg/mouse of twelve SWCNTs or MWCNTs of different properties. Neutrophil influx and DNA damage were assessed on days 1 and 28 post-exposure. Genome microarrays and various bioinformatics and statistical methods were used to identify the biological processes, pathways and functions altered post-exposure to CNTs. All CNTs were ranked for their potency to induce transcriptional perturbation using benchmark dose modelling. All CNTs induced tissue inflammation. MWCNTs were more genotoxic than SWCNTs. Transcriptomics analysis showed similar responses across CNTs at the pathway level at the high dose, which included the perturbation of inflammatory, cellular stress, metabolism, and DNA damage responses. Of all CNTs, one pristine SWCNT was found to be the most potent and potentially fibrogenic, so it should be prioritized for further toxicity testing.

AB - Single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) are nanomaterials with one or multiple layers of carbon sheets. While it is suggested that various properties influence their toxicity, the specific mechanisms are not completely known. This study was aimed to determine if single or multi-walled structures and surface functionalization influence pulmonary toxicity and to identify the underlying mechanisms of toxicity. Female C57BL/6J BomTac mice were exposed to a single dose of 6, 18, or 54 μg/mouse of twelve SWCNTs or MWCNTs of different properties. Neutrophil influx and DNA damage were assessed on days 1 and 28 post-exposure. Genome microarrays and various bioinformatics and statistical methods were used to identify the biological processes, pathways and functions altered post-exposure to CNTs. All CNTs were ranked for their potency to induce transcriptional perturbation using benchmark dose modelling. All CNTs induced tissue inflammation. MWCNTs were more genotoxic than SWCNTs. Transcriptomics analysis showed similar responses across CNTs at the pathway level at the high dose, which included the perturbation of inflammatory, cellular stress, metabolism, and DNA damage responses. Of all CNTs, one pristine SWCNT was found to be the most potent and potentially fibrogenic, so it should be prioritized for further toxicity testing.

KW - carbon nanotubes

KW - fibrosis

KW - functionalization

KW - genotoxicity

KW - inflammation

KW - nanomaterials

KW - pulmonary toxicity

KW - transcriptomics

U2 - 10.3390/nano13061059

DO - 10.3390/nano13061059

M3 - Journal article

C2 - 36985953

AN - SCOPUS:85151534082

VL - 13

JO - Journal of Nanomaterials

JF - Journal of Nanomaterials

SN - 1687-4110

IS - 6

M1 - 1059

ER -