TY - JOUR

T1 - Experimental osteo-fluorosis in the domestic pig

T2 - a histomorphometric study of vertebral trabecular bone

AU - Kragstrup, J

AU - Richards, A

AU - Fejerskov, O

PY - 1984/6

Y1 - 1984/6

N2 - The experiment aimed at describing effects of fluoride on structure and remodeling of vertebral trabecular bone in pigs. Eight animals receiving a supplement of 2 mg F-/kg b.w. per day from age eight to 14 mo were compared with eight controls. Plasma fluoride increased from 0.7 +/- 0.1 microM/1 to 12.7 +/- 2.0 microM/1 in pigs receiving fluoride. At slaughter, the concentration of fluoride in dry fat-free bone was 149.3 +/- 10.5 mM/kg for fluorotic animals and 9.5 +/- 0.9 mM/kg for controls. Morphologic changes were assessed in un-decalcified specimens of the fourth lumbar vertebra by quantitative histology using fluorochromes as intra-vital tissue time markers. The volume of trabecular bone tissue (bone + marrow) was unchanged in fluorotic animals, but the volume density of bone was increased by 17%. Surface densities of cancellous bone were almost unchanged, whereas the thickness of trabeculae increased in fluorosis. Fluoride enhanced remodeling of trabecular surfaces: The fraction of surface occupied by resorption lacunae increased 40%, and the formative surface approximately 30%. No changes were demonstrated at surface points undergoing formation: Osteoid thickness, calcification rate, lamellar thickness, and completed wall thickness were normal. It is concluded that the observed findings cannot be explained by fluoride-induced changes in a single cell. Fluoride appears to affect all cells involved in remodeling by direct or indirect mechanisms.

AB - The experiment aimed at describing effects of fluoride on structure and remodeling of vertebral trabecular bone in pigs. Eight animals receiving a supplement of 2 mg F-/kg b.w. per day from age eight to 14 mo were compared with eight controls. Plasma fluoride increased from 0.7 +/- 0.1 microM/1 to 12.7 +/- 2.0 microM/1 in pigs receiving fluoride. At slaughter, the concentration of fluoride in dry fat-free bone was 149.3 +/- 10.5 mM/kg for fluorotic animals and 9.5 +/- 0.9 mM/kg for controls. Morphologic changes were assessed in un-decalcified specimens of the fourth lumbar vertebra by quantitative histology using fluorochromes as intra-vital tissue time markers. The volume of trabecular bone tissue (bone + marrow) was unchanged in fluorotic animals, but the volume density of bone was increased by 17%. Surface densities of cancellous bone were almost unchanged, whereas the thickness of trabeculae increased in fluorosis. Fluoride enhanced remodeling of trabecular surfaces: The fraction of surface occupied by resorption lacunae increased 40%, and the formative surface approximately 30%. No changes were demonstrated at surface points undergoing formation: Osteoid thickness, calcification rate, lamellar thickness, and completed wall thickness were normal. It is concluded that the observed findings cannot be explained by fluoride-induced changes in a single cell. Fluoride appears to affect all cells involved in remodeling by direct or indirect mechanisms.

KW - Animals

KW - Bone Resorption/etiology

KW - Female

KW - Fluoride Poisoning/complications

KW - Fluorides/adverse effects

KW - Lumbar Vertebrae/drug effects

KW - Osteoclasts/cytology

KW - Osteomalacia/etiology

KW - Swine

KW - Tetracycline

U2 - 10.1177/00220345840630061401

DO - 10.1177/00220345840630061401

M3 - Journal article

C2 - 6588072

VL - 63

SP - 885

EP - 889

JO - Advances in dental research

JF - Advances in dental research

SN - 0895-9374

IS - 6

ER -