Temporal pattern of mutations in the knockdown resistance (kdr) gene of Aedes aegypti mosquitoes sampled from Southern Taiwan
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Temporal pattern of mutations in the knockdown resistance (kdr) gene of Aedes aegypti mosquitoes sampled from Southern Taiwan. / Biduda, Sandrine; Lin, Chia-Hsien; Saleh, Fatma; Konradsen, Flemming; Hansson, Helle; Schiøler, Karin L; Alifrangis, Michael.
I: American Journal of Tropical Medicine and Hygiene, Bind 101, Nr. 5, 2019, s. 973-975.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Temporal pattern of mutations in the knockdown resistance (kdr) gene of Aedes aegypti mosquitoes sampled from Southern Taiwan
AU - Biduda, Sandrine
AU - Lin, Chia-Hsien
AU - Saleh, Fatma
AU - Konradsen, Flemming
AU - Hansson, Helle
AU - Schiøler, Karin L
AU - Alifrangis, Michael
PY - 2019
Y1 - 2019
N2 - Aedes mosquitoes are the principal dengue vector in Taiwan, where the use of insecticides is a key element in the national control strategy. However, control efforts are constrained by the development of resistance to most insecticides, including pyrethroids. In this study, mutations in the voltage-gated sodium channel (VGSC) gene resulting in knockdown resistance (kdr) were examined in Aedes aegypti. Fragments of the VGSC gene were PCR-amplified followed by restriction fragment length polymorphism analysis in samples from various settings in Southern Taiwan covering dry and wet seasons from 2013 to 2015. Three kdr mutations were identified: V1023G, D1794Y, and F1534C, with observed frequencies of 0.36, 0.55, and 0.33, respectively, in the dry season of 2013-2014. Exploring for temporal changes, the most important observation was the 1534C allele frequency increment in the following season to 0.60 (P < 0.05). This study suggests that continued insecticide pressure is driving the mutational changes, although the selection is ambiguous in the mosquito population.
AB - Aedes mosquitoes are the principal dengue vector in Taiwan, where the use of insecticides is a key element in the national control strategy. However, control efforts are constrained by the development of resistance to most insecticides, including pyrethroids. In this study, mutations in the voltage-gated sodium channel (VGSC) gene resulting in knockdown resistance (kdr) were examined in Aedes aegypti. Fragments of the VGSC gene were PCR-amplified followed by restriction fragment length polymorphism analysis in samples from various settings in Southern Taiwan covering dry and wet seasons from 2013 to 2015. Three kdr mutations were identified: V1023G, D1794Y, and F1534C, with observed frequencies of 0.36, 0.55, and 0.33, respectively, in the dry season of 2013-2014. Exploring for temporal changes, the most important observation was the 1534C allele frequency increment in the following season to 0.60 (P < 0.05). This study suggests that continued insecticide pressure is driving the mutational changes, although the selection is ambiguous in the mosquito population.
U2 - 10.4269/ajtmh.19-0289
DO - 10.4269/ajtmh.19-0289
M3 - Journal article
C2 - 31516108
VL - 101
SP - 973
EP - 975
JO - Journal. National Malaria Society
JF - Journal. National Malaria Society
SN - 0002-9637
IS - 5
ER -
ID: 227412470