Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa
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Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa. / Blomberg Jensen, Martin; Bjerrum, Poul J; Jessen, Torben E; Nielsen, John E; Joensen, Ulla N; Olesen, Inge A; Petersen, Jørgen H; Juul, Anders; Dissing, Steen; Jørgensen, Niels.
I: Human Reproduction, Bind 26, Nr. 6, 01.06.2011, s. 1307-17.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa
AU - Blomberg Jensen, Martin
AU - Bjerrum, Poul J
AU - Jessen, Torben E
AU - Nielsen, John E
AU - Joensen, Ulla N
AU - Olesen, Inge A
AU - Petersen, Jørgen H
AU - Juul, Anders
AU - Dissing, Steen
AU - Jørgensen, Niels
PY - 2011/6/1
Y1 - 2011/6/1
N2 - BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.
AB - BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.
U2 - 10.1093/humrep/der059
DO - 10.1093/humrep/der059
M3 - Journal article
C2 - 21427118
VL - 26
SP - 1307
EP - 1317
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 6
ER -
ID: 33543720