Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle
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Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle. / Mikkelsen, Ulla Ramer; Helmark, Ida Carøe; Kjaer, Michael; Langberg, Henning.
I: Journal of Applied Physiology, Bind 104, Nr. 2, 2008, s. 534-537.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle
AU - Mikkelsen, Ulla Ramer
AU - Helmark, Ida Carøe
AU - Kjaer, Michael
AU - Langberg, Henning
N1 - Times Cited: 0ArticleEnglishLangberg, HBispebjerg Hosp, Inst Sports Med, Bispebjerg Hosp, DK-2400 Copenhagen, DenmarkCited References Count: 18260POAMER PHYSIOLOGICAL SOC9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USABETHESDA
PY - 2008
Y1 - 2008
N2 - Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E(2) concentration by approximately 30-50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis.
AB - Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E(2) concentration by approximately 30-50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis.
KW - Adult
KW - Anti-Inflammatory Agents, Non-Steroidal
KW - Catheters, Indwelling
KW - Cyclooxygenase Inhibitors
KW - Dinoprostone
KW - Down-Regulation
KW - Exercise
KW - Humans
KW - Indomethacin
KW - Infusions, Parenteral
KW - Male
KW - Microdialysis
KW - Muscle Contraction
KW - Quadriceps Muscle
KW - Recovery of Function
KW - Time Factors
U2 - 10.1152/japplphysiol.01016.2007
DO - 10.1152/japplphysiol.01016.2007
M3 - Journal article
C2 - 18079272
VL - 104
SP - 534
EP - 537
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 2
ER -
ID: 10454375