Preliminary interlaboratory comparison of the ex vivo dual human placental perfusion system
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Preliminary interlaboratory comparison of the ex vivo dual human placental perfusion system. / Myllynen, Päivi; Mathiesen, Line; Weimer, Marc; Annola, Kirsi; Immonen, Elina; Karttunen, Vesa; Kummu, Maria; Mørck, Thit Juul; Nielsen, Jeanette Kolstrup Søgaard; Knudsen, Lisbeth E.; Vähäkangas, Kirsi.
I: Reproductive Toxicology, Bind 30, 2010, s. 94-102.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Preliminary interlaboratory comparison of the ex vivo dual human placental perfusion system
AU - Myllynen, Päivi
AU - Mathiesen, Line
AU - Weimer, Marc
AU - Annola, Kirsi
AU - Immonen, Elina
AU - Karttunen, Vesa
AU - Kummu, Maria
AU - Mørck, Thit Juul
AU - Nielsen, Jeanette Kolstrup Søgaard
AU - Knudsen, Lisbeth E.
AU - Vähäkangas, Kirsi
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010
Y1 - 2010
N2 - As a part of EU-project ReProTect, a comparison of the dual re-circulating human placental perfusion system was carried out, by two independent research groups. The detailed placental transfer data of model compounds [antipyrine, benzo(a)pyrene, PhIP (2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine) and IQ (2-amino-3-methylimidazo(4,5-f)quinoline] has been/will be published separately. For this project, a comparative re-analysis was done, by curve fitting the data and calculating two endpoints: AUC(120), defined as the area under the curve between time 0 and time 120min and as t(0.5), defined as the time when the fetal to maternal concentration ratio is expected to be 0.5. The transport of the compounds from maternal to fetal circulation across the perfused placenta could be ranked in the order of antipyrine>IQ>PhIP in terms of both t(0.5) and AUC(120) by both partners. For benzo(a)pyrene the curve fitting failed. These prevalidation results give confidence for harmonization of the placental perfusion system to be used as one of the test methods in a panel for reproductive toxicology to model placental transfer in humans.
AB - As a part of EU-project ReProTect, a comparison of the dual re-circulating human placental perfusion system was carried out, by two independent research groups. The detailed placental transfer data of model compounds [antipyrine, benzo(a)pyrene, PhIP (2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine) and IQ (2-amino-3-methylimidazo(4,5-f)quinoline] has been/will be published separately. For this project, a comparative re-analysis was done, by curve fitting the data and calculating two endpoints: AUC(120), defined as the area under the curve between time 0 and time 120min and as t(0.5), defined as the time when the fetal to maternal concentration ratio is expected to be 0.5. The transport of the compounds from maternal to fetal circulation across the perfused placenta could be ranked in the order of antipyrine>IQ>PhIP in terms of both t(0.5) and AUC(120) by both partners. For benzo(a)pyrene the curve fitting failed. These prevalidation results give confidence for harmonization of the placental perfusion system to be used as one of the test methods in a panel for reproductive toxicology to model placental transfer in humans.
U2 - 10.1016/j.reprotox.2010.04.006
DO - 10.1016/j.reprotox.2010.04.006
M3 - Journal article
C2 - 20434538
VL - 30
SP - 94
EP - 102
JO - Reproductive Toxicology
JF - Reproductive Toxicology
SN - 0890-6238
ER -
ID: 20568969