Measurement of salivary cortisol--effects of replacing polyester with cotton and switching antibody
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Measurement of salivary cortisol--effects of replacing polyester with cotton and switching antibody. / Hansen, Ase Marie; Garde, Anne Helene; Persson, Roger; Hansen, Åse Marie.
I: Scandinavian Journal of Clinical & Laboratory Investigation, Bind 68, Nr. 8, 2008, s. 826-829.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Measurement of salivary cortisol--effects of replacing polyester with cotton and switching antibody
AU - Hansen, Ase Marie
AU - Garde, Anne Helene
AU - Persson, Roger
AU - Hansen, Åse Marie
PY - 2008
Y1 - 2008
N2 - Stable performance between-runs are essential in longitudinal studies and when different studies are being compared. However, changes in analytical kits and laboratory material occur and have the potential to threaten analytical stability. In the present case, we examined how salivary cortisol measurements in our laboratory were affected by: 1) changes in the tampon material and 2) changes in the antibody of the analytical kit. In study 1, saliva from healthy subjects (n = 19) was split and spiked to Salivette polyester and cotton tampons, respectively, and treated as ordinary samples before being analysed for cortisol using a Spectria RIA kit for cortisol. In study 2, 68 anonymous saliva samples were analysed with the Spectria Cortisol RIA kit both before and after the manufacturer changed the antibody. The change from polyester to cotton tampons reduced the measured concentration of salivary cortisol by 62 %. A difference of 12 % between the two runs with different antibodies could not be attributed to differences in storage or in thawing and freezing of samples. To conclude, both a change in the material of the Salivette used for collecting saliva samples as well as a change of antibody in a kit can have a major impact on measurements, as illustrated here for concentrations of cortisol in saliva. It is therefore recommended always to check that the analysis stays in statistical control in one's own laboratory when changes are made, even if the manufacturer reports that the changes should have no effects.
AB - Stable performance between-runs are essential in longitudinal studies and when different studies are being compared. However, changes in analytical kits and laboratory material occur and have the potential to threaten analytical stability. In the present case, we examined how salivary cortisol measurements in our laboratory were affected by: 1) changes in the tampon material and 2) changes in the antibody of the analytical kit. In study 1, saliva from healthy subjects (n = 19) was split and spiked to Salivette polyester and cotton tampons, respectively, and treated as ordinary samples before being analysed for cortisol using a Spectria RIA kit for cortisol. In study 2, 68 anonymous saliva samples were analysed with the Spectria Cortisol RIA kit both before and after the manufacturer changed the antibody. The change from polyester to cotton tampons reduced the measured concentration of salivary cortisol by 62 %. A difference of 12 % between the two runs with different antibodies could not be attributed to differences in storage or in thawing and freezing of samples. To conclude, both a change in the material of the Salivette used for collecting saliva samples as well as a change of antibody in a kit can have a major impact on measurements, as illustrated here for concentrations of cortisol in saliva. It is therefore recommended always to check that the analysis stays in statistical control in one's own laboratory when changes are made, even if the manufacturer reports that the changes should have no effects.
KW - Antibodies
KW - Gossypium
KW - Humans
KW - Hydrocortisone
KW - Polyesters
KW - Radioimmunoassay
KW - Saliva
U2 - 10.1080/00365510802056207
DO - 10.1080/00365510802056207
M3 - Journal article
C2 - 18609107
VL - 68
SP - 826
EP - 829
JO - Scandinavian Journal of Clinical & Laboratory Investigation
JF - Scandinavian Journal of Clinical & Laboratory Investigation
SN - 0036-5513
IS - 8
ER -
ID: 10757922