Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC)

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Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC). / Sarkodie, F.; Hassall, O.; Owusu-Dabo, E.; Owusu-Ofori, S.; Bates, I.; Bygbjerg, I. C.; Owusu-Ofori, A.; Harritshøj, L. H.; Ullum, H.

I: Transfusion Medicine, Bind 27, Nr. 1, 02.2017, s. 52–59.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sarkodie, F, Hassall, O, Owusu-Dabo, E, Owusu-Ofori, S, Bates, I, Bygbjerg, IC, Owusu-Ofori, A, Harritshøj, LH & Ullum, H 2017, 'Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC)', Transfusion Medicine, bind 27, nr. 1, s. 52–59. https://doi.org/10.1111/tme.12363

APA

Sarkodie, F., Hassall, O., Owusu-Dabo, E., Owusu-Ofori, S., Bates, I., Bygbjerg, I. C., Owusu-Ofori, A., Harritshøj, L. H., & Ullum, H. (2017). Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC). Transfusion Medicine, 27(1), 52–59. https://doi.org/10.1111/tme.12363

Vancouver

Sarkodie F, Hassall O, Owusu-Dabo E, Owusu-Ofori S, Bates I, Bygbjerg IC o.a. Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC). Transfusion Medicine. 2017 feb.;27(1):52–59. https://doi.org/10.1111/tme.12363

Author

Sarkodie, F. ; Hassall, O. ; Owusu-Dabo, E. ; Owusu-Ofori, S. ; Bates, I. ; Bygbjerg, I. C. ; Owusu-Ofori, A. ; Harritshøj, L. H. ; Ullum, H. / Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC). I: Transfusion Medicine. 2017 ; Bind 27, Nr. 1. s. 52–59.

Bibtex

@article{1b3d1df1ee0c4ae8927d8fc54b341df3,
title = "Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC)",
abstract = "BACKGROUND: Syphilis testing conventionally relies on a combination of non-treponemal and treponemal tests. The primary objective of this study was to describe the positive predictive value (PPV) of a screening algorithm in a combination of a treponemal rapid diagnostic test (RDT) and rapid plasma reagin (RPR) test at Komfo Anokye Teaching Hospital (KATH), Ghana.MATERIALS AND METHODS: From February 2014 to January 2015, 5 mL of venous blood samples were taken from 16 016 blood donors and tested with a treponemal RDT; 5 mL of venous blood was taken from 526 consenting initial syphilis sero-reactive blood donors. These RDT reactive samples were confirmed with an algorithm, applying the Vitros({\textregistered}) /Abbott-Architect({\textregistered}) algorithm as gold standard.RESULTS: A total of 478 of 526 RDT reactive donors were confirmed positive for syphilis, making a PPV of 90·9%. Of the 172 (32·7%) donors who were also RPR positive, 167 were confirmed, resulting in a PPV of 97·1%. The PPV of the combined RDT and RPR (suspected active syphilis) testing algorithm was highest among donors at an enhanced risk of syphilis, family/replacement donors (99·9%), and among voluntary donors above 25 years (98·6%).DISCUSSION: Screening of blood donors by combining syphilis RDT and RPR with relatively good PPV may provide a reasonable technology for LMIC that has a limited capacity for testing and can contribute to the improvement of blood safety with a minimal loss of donors.",
author = "F. Sarkodie and O. Hassall and E. Owusu-Dabo and S. Owusu-Ofori and I. Bates and Bygbjerg, {I. C.} and A. Owusu-Ofori and Harritsh{\o}j, {L. H.} and H. Ullum",
note = "{\textcopyright} 2016 British Blood Transfusion Society.",
year = "2017",
month = feb,
doi = "10.1111/tme.12363",
language = "English",
volume = "27",
pages = "52–59",
journal = "Transfusion Medicine",
issn = "0958-7578",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Improving the screening of blood donors with syphilis rapid diagnostic test (RDT) and rapid plasma reagin (RPR) in low- and middle-income countries (LMIC)

AU - Sarkodie, F.

AU - Hassall, O.

AU - Owusu-Dabo, E.

AU - Owusu-Ofori, S.

AU - Bates, I.

AU - Bygbjerg, I. C.

AU - Owusu-Ofori, A.

AU - Harritshøj, L. H.

AU - Ullum, H.

N1 - © 2016 British Blood Transfusion Society.

PY - 2017/2

Y1 - 2017/2

N2 - BACKGROUND: Syphilis testing conventionally relies on a combination of non-treponemal and treponemal tests. The primary objective of this study was to describe the positive predictive value (PPV) of a screening algorithm in a combination of a treponemal rapid diagnostic test (RDT) and rapid plasma reagin (RPR) test at Komfo Anokye Teaching Hospital (KATH), Ghana.MATERIALS AND METHODS: From February 2014 to January 2015, 5 mL of venous blood samples were taken from 16 016 blood donors and tested with a treponemal RDT; 5 mL of venous blood was taken from 526 consenting initial syphilis sero-reactive blood donors. These RDT reactive samples were confirmed with an algorithm, applying the Vitros(®) /Abbott-Architect(®) algorithm as gold standard.RESULTS: A total of 478 of 526 RDT reactive donors were confirmed positive for syphilis, making a PPV of 90·9%. Of the 172 (32·7%) donors who were also RPR positive, 167 were confirmed, resulting in a PPV of 97·1%. The PPV of the combined RDT and RPR (suspected active syphilis) testing algorithm was highest among donors at an enhanced risk of syphilis, family/replacement donors (99·9%), and among voluntary donors above 25 years (98·6%).DISCUSSION: Screening of blood donors by combining syphilis RDT and RPR with relatively good PPV may provide a reasonable technology for LMIC that has a limited capacity for testing and can contribute to the improvement of blood safety with a minimal loss of donors.

AB - BACKGROUND: Syphilis testing conventionally relies on a combination of non-treponemal and treponemal tests. The primary objective of this study was to describe the positive predictive value (PPV) of a screening algorithm in a combination of a treponemal rapid diagnostic test (RDT) and rapid plasma reagin (RPR) test at Komfo Anokye Teaching Hospital (KATH), Ghana.MATERIALS AND METHODS: From February 2014 to January 2015, 5 mL of venous blood samples were taken from 16 016 blood donors and tested with a treponemal RDT; 5 mL of venous blood was taken from 526 consenting initial syphilis sero-reactive blood donors. These RDT reactive samples were confirmed with an algorithm, applying the Vitros(®) /Abbott-Architect(®) algorithm as gold standard.RESULTS: A total of 478 of 526 RDT reactive donors were confirmed positive for syphilis, making a PPV of 90·9%. Of the 172 (32·7%) donors who were also RPR positive, 167 were confirmed, resulting in a PPV of 97·1%. The PPV of the combined RDT and RPR (suspected active syphilis) testing algorithm was highest among donors at an enhanced risk of syphilis, family/replacement donors (99·9%), and among voluntary donors above 25 years (98·6%).DISCUSSION: Screening of blood donors by combining syphilis RDT and RPR with relatively good PPV may provide a reasonable technology for LMIC that has a limited capacity for testing and can contribute to the improvement of blood safety with a minimal loss of donors.

U2 - 10.1111/tme.12363

DO - 10.1111/tme.12363

M3 - Journal article

C2 - 27723157

VL - 27

SP - 52

EP - 59

JO - Transfusion Medicine

JF - Transfusion Medicine

SN - 0958-7578

IS - 1

ER -

ID: 171651206