Comparison of tuberculin skin test and new specific blood test in tuberculosis contacts
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Comparison of tuberculin skin test and new specific blood test in tuberculosis contacts. / Brock, Inger; Weldingh, Karin; Lillebaek, Troels; Follmann, Frank; Andersen, Peter.
I: American Journal of Respiratory and Critical Care Medicine, Bind 170, Nr. 1, 01.07.2004, s. 65-69.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparison of tuberculin skin test and new specific blood test in tuberculosis contacts
AU - Brock, Inger
AU - Weldingh, Karin
AU - Lillebaek, Troels
AU - Follmann, Frank
AU - Andersen, Peter
PY - 2004/7/1
Y1 - 2004/7/1
N2 - The tuberculin skin test used to detect latent Mycobacterium tuberculosis infection has many drawbacks, and a new diagnostic test for latent tuberculosis (QuantiFERON-TB [QTF-TB]) has recently been introduced. This test measures the production of IFN-γ in whole blood upon stimulation with purified protein derivative (PPD). The QTF-TB test addresses the operational problems with the tuberculin skin test, but, as the test is based on PPD, it still has a low specificity in populations vaccinated with the Bacile Calmette-Guérin (BCG) vaccine. We have modified the test to include the antigens ESAT-6 and CFP-10, which are not present in BCG vaccine strains or the vast majority of nontuberculous mycobacteria. This test was used to detect infection in contacts in a tuberculosis outbreak at a Danish high school. The majority of the contacts were BCG-unvaccinated, which allowed a direct comparison of the skin test and the novel blood test in individuals whose skin test was not confounded by vaccination. An excellent agreement between the two tests was found (94%, K value 0.866), and in contrast to the blood test based on PPD, the novel blood test was not influenced by the vaccination status of the subjects tested.
AB - The tuberculin skin test used to detect latent Mycobacterium tuberculosis infection has many drawbacks, and a new diagnostic test for latent tuberculosis (QuantiFERON-TB [QTF-TB]) has recently been introduced. This test measures the production of IFN-γ in whole blood upon stimulation with purified protein derivative (PPD). The QTF-TB test addresses the operational problems with the tuberculin skin test, but, as the test is based on PPD, it still has a low specificity in populations vaccinated with the Bacile Calmette-Guérin (BCG) vaccine. We have modified the test to include the antigens ESAT-6 and CFP-10, which are not present in BCG vaccine strains or the vast majority of nontuberculous mycobacteria. This test was used to detect infection in contacts in a tuberculosis outbreak at a Danish high school. The majority of the contacts were BCG-unvaccinated, which allowed a direct comparison of the skin test and the novel blood test in individuals whose skin test was not confounded by vaccination. An excellent agreement between the two tests was found (94%, K value 0.866), and in contrast to the blood test based on PPD, the novel blood test was not influenced by the vaccination status of the subjects tested.
KW - Bacile Calmette-Guérin vaccine
KW - Latent infection
KW - Tuberculin skin test
KW - Tuberculosis
KW - Whole blood diagnostic
UR - http://www.scopus.com/inward/record.url?scp=3242881927&partnerID=8YFLogxK
U2 - 10.1164/rccm.200402-232oc
DO - 10.1164/rccm.200402-232oc
M3 - Journal article
C2 - 15087297
AN - SCOPUS:3242881927
VL - 170
SP - 65
EP - 69
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
SN - 0003-0805
IS - 1
ER -
ID: 247165608