Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka

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Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka. / Schousboe, Mette L; Rajakaruna, Rupika S; Amerasinghe, Priyanie H; Konradsen, Flemming; Ord, Rosalynn; Pearce, Richard; Bygbjerg, Ib C; Roper, Cally; Alifrangis, Michael.

I: American Journal of Tropical Medicine and Hygiene, Bind 85, Nr. 6, 01.12.2011, s. 994-1001.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Schousboe, ML, Rajakaruna, RS, Amerasinghe, PH, Konradsen, F, Ord, R, Pearce, R, Bygbjerg, IC, Roper, C & Alifrangis, M 2011, 'Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka', American Journal of Tropical Medicine and Hygiene, bind 85, nr. 6, s. 994-1001. https://doi.org/10.4269/ajtmh.2011.11-0338

APA

Schousboe, M. L., Rajakaruna, R. S., Amerasinghe, P. H., Konradsen, F., Ord, R., Pearce, R., Bygbjerg, I. C., Roper, C., & Alifrangis, M. (2011). Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka. American Journal of Tropical Medicine and Hygiene, 85(6), 994-1001. https://doi.org/10.4269/ajtmh.2011.11-0338

Vancouver

Schousboe ML, Rajakaruna RS, Amerasinghe PH, Konradsen F, Ord R, Pearce R o.a. Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka. American Journal of Tropical Medicine and Hygiene. 2011 dec. 1;85(6):994-1001. https://doi.org/10.4269/ajtmh.2011.11-0338

Author

Schousboe, Mette L ; Rajakaruna, Rupika S ; Amerasinghe, Priyanie H ; Konradsen, Flemming ; Ord, Rosalynn ; Pearce, Richard ; Bygbjerg, Ib C ; Roper, Cally ; Alifrangis, Michael. / Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka. I: American Journal of Tropical Medicine and Hygiene. 2011 ; Bind 85, Nr. 6. s. 994-1001.

Bibtex

@article{090609bc8da147bdbf119505c6394150,
title = "Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka",
abstract = "Abstract. The geographical distribution of genetic variation in Plasmodium vivax samples (N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein-3a (Pvmsp-3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp-3a, 11 alleles were found with an expected heterozygosity (H(e)) of 0.81, whereas at m1501 and m3502, 24 alleles (H(e) = 0.85) and 8 alleles (H(e) = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H(e) = 0.95). Calculating the pairwise fixation index (F(ST)) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka.",
author = "Schousboe, {Mette L} and Rajakaruna, {Rupika S} and Amerasinghe, {Priyanie H} and Flemming Konradsen and Rosalynn Ord and Richard Pearce and Bygbjerg, {Ib C} and Cally Roper and Michael Alifrangis",
year = "2011",
month = dec,
day = "1",
doi = "10.4269/ajtmh.2011.11-0338",
language = "English",
volume = "85",
pages = "994--1001",
journal = "Journal. National Malaria Society",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "6",

}

RIS

TY - JOUR

T1 - Analysis of Polymorphisms in the Merozoite Surface Protein-3a Gene and Two Microsatellite Loci in Sri Lankan Plasmodium vivax: Evidence of Population Substructure in Sri Lanka

AU - Schousboe, Mette L

AU - Rajakaruna, Rupika S

AU - Amerasinghe, Priyanie H

AU - Konradsen, Flemming

AU - Ord, Rosalynn

AU - Pearce, Richard

AU - Bygbjerg, Ib C

AU - Roper, Cally

AU - Alifrangis, Michael

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Abstract. The geographical distribution of genetic variation in Plasmodium vivax samples (N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein-3a (Pvmsp-3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp-3a, 11 alleles were found with an expected heterozygosity (H(e)) of 0.81, whereas at m1501 and m3502, 24 alleles (H(e) = 0.85) and 8 alleles (H(e) = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H(e) = 0.95). Calculating the pairwise fixation index (F(ST)) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka.

AB - Abstract. The geographical distribution of genetic variation in Plasmodium vivax samples (N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein-3a (Pvmsp-3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp-3a, 11 alleles were found with an expected heterozygosity (H(e)) of 0.81, whereas at m1501 and m3502, 24 alleles (H(e) = 0.85) and 8 alleles (H(e) = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H(e) = 0.95). Calculating the pairwise fixation index (F(ST)) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka.

U2 - 10.4269/ajtmh.2011.11-0338

DO - 10.4269/ajtmh.2011.11-0338

M3 - Journal article

C2 - 22144433

VL - 85

SP - 994

EP - 1001

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

SN - 0002-9637

IS - 6

ER -

ID: 35453777