A method for preparing and staining histological sections containing titanium implants for light microscopy
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A method for preparing and staining histological sections containing titanium implants for light microscopy. / Gotfredsen, K.; Budtz-Jörgensen, E.; Jensen, L. Nimb.
I: Biotechnic and Histochemistry, Bind 64, Nr. 3, 01.01.1989, s. 121-127.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A method for preparing and staining histological sections containing titanium implants for light microscopy
AU - Gotfredsen, K.
AU - Budtz-Jörgensen, E.
AU - Jensen, L. Nimb
PY - 1989/1/1
Y1 - 1989/1/1
N2 - An improved method for preparing and staining ground tissue-implant sections for light microscopy is presented. Undecalcified tissue blocks with titanium implants were dehydrated in an ascending series of ethanol and stained in toto with basic fuchsin. Specimens were infiltrated and embedded in methyl methacrylate and sections were prepared using a cutting-grinding-system. The polished surface was counterstained with light green or anilin blue. Light polymerizing resin was used as slide mounting medium and for mounting the coverglass. The sections obtained were 10-15 μm thick with tissue architecture which clearly differentiated structures at the tissue-implant interface. The method was very useful for computer assisted morphometric analysis.
AB - An improved method for preparing and staining ground tissue-implant sections for light microscopy is presented. Undecalcified tissue blocks with titanium implants were dehydrated in an ascending series of ethanol and stained in toto with basic fuchsin. Specimens were infiltrated and embedded in methyl methacrylate and sections were prepared using a cutting-grinding-system. The polished surface was counterstained with light green or anilin blue. Light polymerizing resin was used as slide mounting medium and for mounting the coverglass. The sections obtained were 10-15 μm thick with tissue architecture which clearly differentiated structures at the tissue-implant interface. The method was very useful for computer assisted morphometric analysis.
UR - http://www.scopus.com/inward/record.url?scp=84907131630&partnerID=8YFLogxK
U2 - 10.3109/10520298909106984
DO - 10.3109/10520298909106984
M3 - Journal article
C2 - 2480002
AN - SCOPUS:84907131630
VL - 64
SP - 121
EP - 127
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
SN - 1052-0295
IS - 3
ER -
ID: 218656968