Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells

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Standard

Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells. / Billestrup, N; Møldrup, A; Serup, P; Mathews, L S; Norstedt, G; Nielsen, Jens Høiriis.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 87, Nr. 18, 09.1990, s. 7210-4.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Billestrup, N, Møldrup, A, Serup, P, Mathews, LS, Norstedt, G & Nielsen, JH 1990, 'Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells', Proceedings of the National Academy of Sciences of the United States of America, bind 87, nr. 18, s. 7210-4.

APA

Billestrup, N., Møldrup, A., Serup, P., Mathews, L. S., Norstedt, G., & Nielsen, J. H. (1990). Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells. Proceedings of the National Academy of Sciences of the United States of America, 87(18), 7210-4.

Vancouver

Billestrup N, Møldrup A, Serup P, Mathews LS, Norstedt G, Nielsen JH. Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells. Proceedings of the National Academy of Sciences of the United States of America. 1990 sep.;87(18):7210-4.

Author

Billestrup, N ; Møldrup, A ; Serup, P ; Mathews, L S ; Norstedt, G ; Nielsen, Jens Høiriis. / Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells. I: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Bind 87, Nr. 18. s. 7210-4.

Bibtex

@article{b974a97e70494d7b926bad4e680bb173,
title = "Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells",
abstract = "The stimulation of insulin biosynthesis in the pancreatic insulinoma cell line RIN5-AH by growth hormone (GH) is initiated by GH binding to specific receptors. To determine whether the recently cloned rat hepatic GH receptor is able to mediate the insulinotropic effect of GH, we have transfected a GH receptor cDNA under the transcriptional control of the human metallothionein promoter into RIN5-AH cells. The transfected cells were found to exhibit an increased expression of GH receptors and to contain a specific GH receptor mRNA that was not expressed in the parent cell line. The expression of GH receptors in one clone (1.24) selected for detailed analysis was increased 2.6-fold compared to untransfected cells. The increased GH receptor expression was accompanied by an increased responsiveness to GH. Thus, the maximal GH-stimulated increase of insulin biosynthesis was 4.1-fold in 1.24 cells compared to 1.9-fold in the nontransfected RIN5-AH cells. The expression of the transfected receptor was stimulated 1.6- and 2.3-fold when cells were cultured in the presence of 25 or 50 microM Zn2+, respectively. The increased expression of the GH receptor by Zn2+ was associated with an increased magnitude of GH-stimulated insulin biosynthesis. A close stoichiometric relationship between the level of receptor expression and the level of GH-stimulated insulin biosynthesis was observed. We conclude from these results that the hepatic GH receptor is able to mediate the effect of GH on insulin biosynthesis in RIN5-AH cells.",
keywords = "Animals, Cell Line, Gene Expression, Genetic Vectors, Growth Hormone, Humans, Insulin, Insulinoma, Kinetics, Pancreatic Neoplasms, Plasmids, Rats, Receptors, Somatotropin, Transfection, Zinc",
author = "N Billestrup and A M{\o}ldrup and P Serup and Mathews, {L S} and G Norstedt and Nielsen, {Jens H{\o}iriis}",
year = "1990",
month = sep,
language = "English",
volume = "87",
pages = "7210--4",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "18",

}

RIS

TY - JOUR

T1 - Introduction of exogenous growth hormone receptors augments growth hormone-responsive insulin biosynthesis in rat insulinoma cells

AU - Billestrup, N

AU - Møldrup, A

AU - Serup, P

AU - Mathews, L S

AU - Norstedt, G

AU - Nielsen, Jens Høiriis

PY - 1990/9

Y1 - 1990/9

N2 - The stimulation of insulin biosynthesis in the pancreatic insulinoma cell line RIN5-AH by growth hormone (GH) is initiated by GH binding to specific receptors. To determine whether the recently cloned rat hepatic GH receptor is able to mediate the insulinotropic effect of GH, we have transfected a GH receptor cDNA under the transcriptional control of the human metallothionein promoter into RIN5-AH cells. The transfected cells were found to exhibit an increased expression of GH receptors and to contain a specific GH receptor mRNA that was not expressed in the parent cell line. The expression of GH receptors in one clone (1.24) selected for detailed analysis was increased 2.6-fold compared to untransfected cells. The increased GH receptor expression was accompanied by an increased responsiveness to GH. Thus, the maximal GH-stimulated increase of insulin biosynthesis was 4.1-fold in 1.24 cells compared to 1.9-fold in the nontransfected RIN5-AH cells. The expression of the transfected receptor was stimulated 1.6- and 2.3-fold when cells were cultured in the presence of 25 or 50 microM Zn2+, respectively. The increased expression of the GH receptor by Zn2+ was associated with an increased magnitude of GH-stimulated insulin biosynthesis. A close stoichiometric relationship between the level of receptor expression and the level of GH-stimulated insulin biosynthesis was observed. We conclude from these results that the hepatic GH receptor is able to mediate the effect of GH on insulin biosynthesis in RIN5-AH cells.

AB - The stimulation of insulin biosynthesis in the pancreatic insulinoma cell line RIN5-AH by growth hormone (GH) is initiated by GH binding to specific receptors. To determine whether the recently cloned rat hepatic GH receptor is able to mediate the insulinotropic effect of GH, we have transfected a GH receptor cDNA under the transcriptional control of the human metallothionein promoter into RIN5-AH cells. The transfected cells were found to exhibit an increased expression of GH receptors and to contain a specific GH receptor mRNA that was not expressed in the parent cell line. The expression of GH receptors in one clone (1.24) selected for detailed analysis was increased 2.6-fold compared to untransfected cells. The increased GH receptor expression was accompanied by an increased responsiveness to GH. Thus, the maximal GH-stimulated increase of insulin biosynthesis was 4.1-fold in 1.24 cells compared to 1.9-fold in the nontransfected RIN5-AH cells. The expression of the transfected receptor was stimulated 1.6- and 2.3-fold when cells were cultured in the presence of 25 or 50 microM Zn2+, respectively. The increased expression of the GH receptor by Zn2+ was associated with an increased magnitude of GH-stimulated insulin biosynthesis. A close stoichiometric relationship between the level of receptor expression and the level of GH-stimulated insulin biosynthesis was observed. We conclude from these results that the hepatic GH receptor is able to mediate the effect of GH on insulin biosynthesis in RIN5-AH cells.

KW - Animals

KW - Cell Line

KW - Gene Expression

KW - Genetic Vectors

KW - Growth Hormone

KW - Humans

KW - Insulin

KW - Insulinoma

KW - Kinetics

KW - Pancreatic Neoplasms

KW - Plasmids

KW - Rats

KW - Receptors, Somatotropin

KW - Transfection

KW - Zinc

M3 - Journal article

C2 - 2205855

VL - 87

SP - 7210

EP - 7214

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 18

ER -

ID: 47973963