Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics. / Nikolajsen, Rikke P; Hansen, Åse Marie; Bro, R.

I: Luminescence, Bind 16, Nr. 2, 2000, s. 91-101.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nikolajsen, RP, Hansen, ÅM & Bro, R 2000, 'Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics', Luminescence, bind 16, nr. 2, s. 91-101. https://doi.org/10.1002/bio.637

APA

Nikolajsen, R. P., Hansen, Å. M., & Bro, R. (2000). Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics. Luminescence, 16(2), 91-101. https://doi.org/10.1002/bio.637

Vancouver

Nikolajsen RP, Hansen ÅM, Bro R. Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics. Luminescence. 2000;16(2):91-101. https://doi.org/10.1002/bio.637

Author

Nikolajsen, Rikke P ; Hansen, Åse Marie ; Bro, R. / Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics. I: Luminescence. 2000 ; Bind 16, Nr. 2. s. 91-101.

Bibtex

@article{6bec2538391349f4bf7696c1ce5aeb68,
title = "Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics",
abstract = "An investigation was conducted on whether the fluorescence spectra of the very similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 and 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH approximately equal to 3) spiked with the catecholamines in the concentration ranges 40--1200 nmol/L and 5.5--18 micromol/L, respectively. Unfold partial least squares regression (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFAC) of the six samples of the smaller set showed that there was no difference between the fluorescence landscapes of adrenaline and noradrenaline. It can be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatter, which overlaps the catecholamine spectra, was shown not to have any influence on the models calculated.",
keywords = "Calibration, Epinephrine, Multivariate Analysis, Norepinephrine, Spectrometry, Fluorescence",
author = "Nikolajsen, {Rikke P} and Hansen, {{\AA}se Marie} and R Bro",
note = "Copyright 2001 John Wiley & Sons, Ltd.",
year = "2000",
doi = "10.1002/bio.637",
language = "English",
volume = "16",
pages = "91--101",
journal = "Luminescence",
issn = "1522-7235",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics

AU - Nikolajsen, Rikke P

AU - Hansen, Åse Marie

AU - Bro, R

N1 - Copyright 2001 John Wiley & Sons, Ltd.

PY - 2000

Y1 - 2000

N2 - An investigation was conducted on whether the fluorescence spectra of the very similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 and 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH approximately equal to 3) spiked with the catecholamines in the concentration ranges 40--1200 nmol/L and 5.5--18 micromol/L, respectively. Unfold partial least squares regression (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFAC) of the six samples of the smaller set showed that there was no difference between the fluorescence landscapes of adrenaline and noradrenaline. It can be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatter, which overlaps the catecholamine spectra, was shown not to have any influence on the models calculated.

AB - An investigation was conducted on whether the fluorescence spectra of the very similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 and 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH approximately equal to 3) spiked with the catecholamines in the concentration ranges 40--1200 nmol/L and 5.5--18 micromol/L, respectively. Unfold partial least squares regression (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFAC) of the six samples of the smaller set showed that there was no difference between the fluorescence landscapes of adrenaline and noradrenaline. It can be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatter, which overlaps the catecholamine spectra, was shown not to have any influence on the models calculated.

KW - Calibration

KW - Epinephrine

KW - Multivariate Analysis

KW - Norepinephrine

KW - Spectrometry, Fluorescence

U2 - 10.1002/bio.637

DO - 10.1002/bio.637

M3 - Journal article

C2 - 11312534

VL - 16

SP - 91

EP - 101

JO - Luminescence

JF - Luminescence

SN - 1522-7235

IS - 2

ER -

ID: 48865608