Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017

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Standard

Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017. / Joensen, Katrine G.; Kiil, Kristoffer; Gantzhorn, Mette R.; Nauerby, Birgitte; Engberg, Jørgen; Holt, Hanne M.; Nielsen, Hans L.; Petersen, Andreas M.; Kuhn, Katrin G.; Sandø, Gudrun; Ethelberg, Steen; Nielsen, Eva M.

I: Emerging Infectious Diseases (Online), Bind 26, Nr. 3, 2020, s. 523-532.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Joensen, KG, Kiil, K, Gantzhorn, MR, Nauerby, B, Engberg, J, Holt, HM, Nielsen, HL, Petersen, AM, Kuhn, KG, Sandø, G, Ethelberg, S & Nielsen, EM 2020, 'Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017', Emerging Infectious Diseases (Online), bind 26, nr. 3, s. 523-532. https://doi.org/10.3201/eid2603.190947

APA

Joensen, K. G., Kiil, K., Gantzhorn, M. R., Nauerby, B., Engberg, J., Holt, H. M., ... Nielsen, E. M. (2020). Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017. Emerging Infectious Diseases (Online), 26(3), 523-532. https://doi.org/10.3201/eid2603.190947

Vancouver

Joensen KG, Kiil K, Gantzhorn MR, Nauerby B, Engberg J, Holt HM o.a. Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017. Emerging Infectious Diseases (Online). 2020;26(3):523-532. https://doi.org/10.3201/eid2603.190947

Author

Joensen, Katrine G. ; Kiil, Kristoffer ; Gantzhorn, Mette R. ; Nauerby, Birgitte ; Engberg, Jørgen ; Holt, Hanne M. ; Nielsen, Hans L. ; Petersen, Andreas M. ; Kuhn, Katrin G. ; Sandø, Gudrun ; Ethelberg, Steen ; Nielsen, Eva M. / Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017. I: Emerging Infectious Diseases (Online). 2020 ; Bind 26, Nr. 3. s. 523-532.

Bibtex

@article{9079c470f84c421d8fe6d95bf3440ab4,
title = "Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017",
abstract = "In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3{\%}) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54{\%}) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.",
author = "Joensen, {Katrine G.} and Kristoffer Kiil and Gantzhorn, {Mette R.} and Birgitte Nauerby and J{\o}rgen Engberg and Holt, {Hanne M.} and Nielsen, {Hans L.} and Petersen, {Andreas M.} and Kuhn, {Katrin G.} and Gudrun Sand{\o} and Steen Ethelberg and Nielsen, {Eva M.}",
year = "2020",
doi = "10.3201/eid2603.190947",
language = "English",
volume = "26",
pages = "523--532",
journal = "Emerging Infectious Diseases (Online)",
issn = "1080-6040",
publisher = "U.S. Department of Health and Human Services Centers for Disease Control and Prevention",
number = "3",

}

RIS

TY - JOUR

T1 - Whole-genome sequencing to detect numerous campylobacter jejuni outbreaks and match patient isolates to sources, Denmark, 2015-2017

AU - Joensen, Katrine G.

AU - Kiil, Kristoffer

AU - Gantzhorn, Mette R.

AU - Nauerby, Birgitte

AU - Engberg, Jørgen

AU - Holt, Hanne M.

AU - Nielsen, Hans L.

AU - Petersen, Andreas M.

AU - Kuhn, Katrin G.

AU - Sandø, Gudrun

AU - Ethelberg, Steen

AU - Nielsen, Eva M.

PY - 2020

Y1 - 2020

N2 - In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3%) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54%) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.

AB - In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3%) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54%) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.

U2 - 10.3201/eid2603.190947

DO - 10.3201/eid2603.190947

M3 - Journal article

C2 - 32091364

AN - SCOPUS:85079910115

VL - 26

SP - 523

EP - 532

JO - Emerging Infectious Diseases (Online)

JF - Emerging Infectious Diseases (Online)

SN - 1080-6040

IS - 3

ER -

ID: 244915054