Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound

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Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound. / Roursgaard, Martin; Poulsen, Steen Seier; Poulsen, Lars K.; Hammer, Maria; Jensen, Keld A.; Utsunomiya, Satoshi; Ewing, Rodney C.; Balic Zunic, Tonci; Nielsen, Gunnar D.; Larsen, Søren T.

I: Human & Experimental Toxicology, Bind 29, Nr. 11, 2010, s. 915-933.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Roursgaard, M, Poulsen, SS, Poulsen, LK, Hammer, M, Jensen, KA, Utsunomiya, S, Ewing, RC, Balic Zunic, T, Nielsen, GD & Larsen, ST 2010, 'Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound', Human & Experimental Toxicology, bind 29, nr. 11, s. 915-933. https://doi.org/10.1177/0960327110363329

APA

Roursgaard, M., Poulsen, S. S., Poulsen, L. K., Hammer, M., Jensen, K. A., Utsunomiya, S., Ewing, R. C., Balic Zunic, T., Nielsen, G. D., & Larsen, S. T. (2010). Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound. Human & Experimental Toxicology, 29(11), 915-933. https://doi.org/10.1177/0960327110363329

Vancouver

Roursgaard M, Poulsen SS, Poulsen LK, Hammer M, Jensen KA, Utsunomiya S o.a. Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound. Human & Experimental Toxicology. 2010;29(11):915-933. https://doi.org/10.1177/0960327110363329

Author

Roursgaard, Martin ; Poulsen, Steen Seier ; Poulsen, Lars K. ; Hammer, Maria ; Jensen, Keld A. ; Utsunomiya, Satoshi ; Ewing, Rodney C. ; Balic Zunic, Tonci ; Nielsen, Gunnar D. ; Larsen, Søren T. / Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound. I: Human & Experimental Toxicology. 2010 ; Bind 29, Nr. 11. s. 915-933.

Bibtex

@article{14dffb6619b54f4cba711daa31a9145a,
title = "Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound",
abstract = "An increasing number of engineered particles, including nanoparticles, are being manufactured, increasing the need for simple low-dose toxicological screening methods. This study aimed to investigate the kinetics of biomarkers related to acute and sub-chronic particle-induced lung inflammation of quartz. Mice were intratracheal instilled with 50 µg of microsized or nanosized quartz. Acute inflammation was assessed 1, 2, 4, 8, 16 or 48 hours post exposure, whereas sub-chronic inflammation was investigated 3 months after exposure. Markers of acute inflammation in the bronchoalveolar lavage fluid (BALF) were neutrophils (PMN), tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1{\ss}, macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC) and total protein, which were all close to maximum 16 hours post instillation. No major differences were seen in the time-response profiles of nano- and micro-sized particles. The potency of the two samples cannot be compared; during the milling process, a substantial part of the quartz was converted to amorphous silica and contaminated with corundum. For screening, BALF PMN, either TNF-a or IL-1{\ss} at 16 hours post instillation may be useful. At 3 months post instillation, KC, PMN and macrophages were elevated. Histology showed no interstitial inflammation three months post instillation. For screening of sub-chronic effects, KC, PMN, macrophages and histopathology is considered sufficient.",
keywords = "Animals, Bronchoalveolar Lavage Fluid, Cytokines, Dose-Response Relationship, Drug, Female, Inhalation Exposure, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Nanoparticles, Neutrophils, Particle Size, Pneumonia, Powder Diffraction, Quartz, Surface Properties, Time Factors, Toxicity Tests, X-Ray Diffraction",
author = "Martin Roursgaard and Poulsen, {Steen Seier} and Poulsen, {Lars K.} and Maria Hammer and Jensen, {Keld A.} and Satoshi Utsunomiya and Ewing, {Rodney C.} and {Balic Zunic}, Tonci and Nielsen, {Gunnar D.} and Larsen, {S{\o}ren T.}",
year = "2010",
doi = "10.1177/0960327110363329",
language = "English",
volume = "29",
pages = "915--933",
journal = "Human and Experimental Toxicology",
issn = "0960-3271",
publisher = "SAGE Publications",
number = "11",

}

RIS

TY - JOUR

T1 - Time-response relationship of nano and micro particle induced lung inflammation. Quartz as reference compound

AU - Roursgaard, Martin

AU - Poulsen, Steen Seier

AU - Poulsen, Lars K.

AU - Hammer, Maria

AU - Jensen, Keld A.

AU - Utsunomiya, Satoshi

AU - Ewing, Rodney C.

AU - Balic Zunic, Tonci

AU - Nielsen, Gunnar D.

AU - Larsen, Søren T.

PY - 2010

Y1 - 2010

N2 - An increasing number of engineered particles, including nanoparticles, are being manufactured, increasing the need for simple low-dose toxicological screening methods. This study aimed to investigate the kinetics of biomarkers related to acute and sub-chronic particle-induced lung inflammation of quartz. Mice were intratracheal instilled with 50 µg of microsized or nanosized quartz. Acute inflammation was assessed 1, 2, 4, 8, 16 or 48 hours post exposure, whereas sub-chronic inflammation was investigated 3 months after exposure. Markers of acute inflammation in the bronchoalveolar lavage fluid (BALF) were neutrophils (PMN), tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1ß, macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC) and total protein, which were all close to maximum 16 hours post instillation. No major differences were seen in the time-response profiles of nano- and micro-sized particles. The potency of the two samples cannot be compared; during the milling process, a substantial part of the quartz was converted to amorphous silica and contaminated with corundum. For screening, BALF PMN, either TNF-a or IL-1ß at 16 hours post instillation may be useful. At 3 months post instillation, KC, PMN and macrophages were elevated. Histology showed no interstitial inflammation three months post instillation. For screening of sub-chronic effects, KC, PMN, macrophages and histopathology is considered sufficient.

AB - An increasing number of engineered particles, including nanoparticles, are being manufactured, increasing the need for simple low-dose toxicological screening methods. This study aimed to investigate the kinetics of biomarkers related to acute and sub-chronic particle-induced lung inflammation of quartz. Mice were intratracheal instilled with 50 µg of microsized or nanosized quartz. Acute inflammation was assessed 1, 2, 4, 8, 16 or 48 hours post exposure, whereas sub-chronic inflammation was investigated 3 months after exposure. Markers of acute inflammation in the bronchoalveolar lavage fluid (BALF) were neutrophils (PMN), tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1ß, macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC) and total protein, which were all close to maximum 16 hours post instillation. No major differences were seen in the time-response profiles of nano- and micro-sized particles. The potency of the two samples cannot be compared; during the milling process, a substantial part of the quartz was converted to amorphous silica and contaminated with corundum. For screening, BALF PMN, either TNF-a or IL-1ß at 16 hours post instillation may be useful. At 3 months post instillation, KC, PMN and macrophages were elevated. Histology showed no interstitial inflammation three months post instillation. For screening of sub-chronic effects, KC, PMN, macrophages and histopathology is considered sufficient.

KW - Animals

KW - Bronchoalveolar Lavage Fluid

KW - Cytokines

KW - Dose-Response Relationship, Drug

KW - Female

KW - Inhalation Exposure

KW - Mice

KW - Mice, Inbred BALB C

KW - Microscopy, Electron, Transmission

KW - Nanoparticles

KW - Neutrophils

KW - Particle Size

KW - Pneumonia

KW - Powder Diffraction

KW - Quartz

KW - Surface Properties

KW - Time Factors

KW - Toxicity Tests

KW - X-Ray Diffraction

U2 - 10.1177/0960327110363329

DO - 10.1177/0960327110363329

M3 - Journal article

C2 - 20237177

VL - 29

SP - 915

EP - 933

JO - Human and Experimental Toxicology

JF - Human and Experimental Toxicology

SN - 0960-3271

IS - 11

ER -

ID: 33717947