TY - CONF

T1 - Prevalence of Virulent Escherichia coli Belonging B1 Phylogroup in Municipal Water Supply in Dhaka, Bangladesh

AU - Ferdous, Jannataul

AU - Rashid, Ridwan Bin

AU - Tulsiani, Suhella

AU - Jensen, Peter Kjaer Mackie

AU - Begum, Anowara

PY - 2016/6

Y1 - 2016/6

N2 - Escherichia coli is a commensal organism of the digestive tracts of manyvertebrates, including humans. Contamination of drinking water withpathogenic E. coli is a serious public health concern. This study focused onthe distribution of phylogenetic groups and virulence gene profile of E. coliisolated from drinking water in Arichpur, a low income area of Dhaka,Bangladesh. The distribution of the phylogroups and virulence genes wereinvestigated in 200 isolates among them 110 isolates were from municipalwater supply system and 90 were from household drinking water. Geneprofile of virulence factors was done based on the presence of eltB, estA,vt1, vt2, eaeA, pCVD432, bfpA, ial, ipaH by PCR. The classification of theisolates into 4/5 major groups (A, B1, B2, D2, D3) was done based on thedistribution of chuA, yjaA and DNA fragment tspE4.C2 genes. Resultsdemonstrated predominance of phylogroup B1 78.5 % (157/200) followedby B2 phylogroup 13% (26/200) and phylogroup D 8.5% (17/200).The geneseltB, estA and eaeA was present in 27.39% (43/157), 37% (74/157), and10.5% (21/157) of B1 phylogroup isolates, respectively. The calculated chisquare value and P-value were 10.23 and 0.001. Therefore, it can beinferred municipal water supply was a greater contributor of pathogenic E.coli from the B1 phylogroup. Usually commensals fall in the Phylogroups Aand B1. The presence of greater number of virulent B1 phylogroup isolatesoriginating from municipal water supply indicates that the supply systemmight be contaminated with virulent E. coli such as enterotoxigenic E. colicarrying mobile genetic elements such as plasmids which might betransferred to the commensal strains

AB - Escherichia coli is a commensal organism of the digestive tracts of manyvertebrates, including humans. Contamination of drinking water withpathogenic E. coli is a serious public health concern. This study focused onthe distribution of phylogenetic groups and virulence gene profile of E. coliisolated from drinking water in Arichpur, a low income area of Dhaka,Bangladesh. The distribution of the phylogroups and virulence genes wereinvestigated in 200 isolates among them 110 isolates were from municipalwater supply system and 90 were from household drinking water. Geneprofile of virulence factors was done based on the presence of eltB, estA,vt1, vt2, eaeA, pCVD432, bfpA, ial, ipaH by PCR. The classification of theisolates into 4/5 major groups (A, B1, B2, D2, D3) was done based on thedistribution of chuA, yjaA and DNA fragment tspE4.C2 genes. Resultsdemonstrated predominance of phylogroup B1 78.5 % (157/200) followedby B2 phylogroup 13% (26/200) and phylogroup D 8.5% (17/200).The geneseltB, estA and eaeA was present in 27.39% (43/157), 37% (74/157), and10.5% (21/157) of B1 phylogroup isolates, respectively. The calculated chisquare value and P-value were 10.23 and 0.001. Therefore, it can beinferred municipal water supply was a greater contributor of pathogenic E.coli from the B1 phylogroup. Usually commensals fall in the Phylogroups Aand B1. The presence of greater number of virulent B1 phylogroup isolatesoriginating from municipal water supply indicates that the supply systemmight be contaminated with virulent E. coli such as enterotoxigenic E. colicarrying mobile genetic elements such as plasmids which might betransferred to the commensal strains

UR - http://www.asm.org/index.php/asm-events/post-meeting-materials

M3 - Poster

T2 - American socierty for microbiology 2016

Y2 - 16 June 2016 through 20 June 2016

ER -