Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans
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Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans. / Crameri, Regina M; Langberg, Henning; Teisner, Børge; Magnusson, Peter; Schrøder, Henrik Daa; Olesen, Jens L; Jensen, Charlotte H; Koskinen, Satu; Suetta, Charlotte; Kjaer, Michael.
I: Matrix Biology, Bind 23, Nr. 4, 2004, s. 259-64.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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T1 - Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans
AU - Crameri, Regina M
AU - Langberg, Henning
AU - Teisner, Børge
AU - Magnusson, Peter
AU - Schrøder, Henrik Daa
AU - Olesen, Jens L
AU - Jensen, Charlotte H
AU - Koskinen, Satu
AU - Suetta, Charlotte
AU - Kjaer, Michael
PY - 2004
Y1 - 2004
N2 - Increases in procollagen processing within skeletal muscle have previously been reported in small animal models only. While indirect measurements in humans have suggested an increase procollagen processing, no intra-skeletal muscle measurements have confirmed these findings. In this study, eight young healthy male subjects performed a single bout of unaccustomed high intensity eccentric exercise on one leg, with the contralateral leg being the control. A significant increase in the muscle interstitial concentration of the N-terminal propeptide of procollagen type I (PINP) was observed (day 0: 1.96 +/- 0.44 ng ml(-1), day 2: 1.94 +/- 0.32 ng ml(-1), day 4: 3.90 +/- 1.03 ng ml(-1), day 8: 7.23 +/- 2.34 ng ml(-1)*, *P <0.05 vs. basal and control) with no change being noted in the control leg. By day 2 post-exercise, an increase in the histological immunoreactivity of PINP and the N-terminal propeptide of procollagen type III (PIIINP) was also shown in the exercising leg only. Further, from day 2 post-exercise, immunoreactivity for tenascin C and reactive macrophages (CD68+ cells) was seen within the perimysial and endomysial connective tissue of the exercising leg only, indicating a high mechanical load and inflammation. This study shows that following a single bout of high intensity eccentric exercise there is an increase in procollagen processing within skeletal muscle in humans.
AB - Increases in procollagen processing within skeletal muscle have previously been reported in small animal models only. While indirect measurements in humans have suggested an increase procollagen processing, no intra-skeletal muscle measurements have confirmed these findings. In this study, eight young healthy male subjects performed a single bout of unaccustomed high intensity eccentric exercise on one leg, with the contralateral leg being the control. A significant increase in the muscle interstitial concentration of the N-terminal propeptide of procollagen type I (PINP) was observed (day 0: 1.96 +/- 0.44 ng ml(-1), day 2: 1.94 +/- 0.32 ng ml(-1), day 4: 3.90 +/- 1.03 ng ml(-1), day 8: 7.23 +/- 2.34 ng ml(-1)*, *P <0.05 vs. basal and control) with no change being noted in the control leg. By day 2 post-exercise, an increase in the histological immunoreactivity of PINP and the N-terminal propeptide of procollagen type III (PIIINP) was also shown in the exercising leg only. Further, from day 2 post-exercise, immunoreactivity for tenascin C and reactive macrophages (CD68+ cells) was seen within the perimysial and endomysial connective tissue of the exercising leg only, indicating a high mechanical load and inflammation. This study shows that following a single bout of high intensity eccentric exercise there is an increase in procollagen processing within skeletal muscle in humans.
KW - Adult
KW - Exercise
KW - Extracellular Matrix Proteins
KW - Humans
KW - Leg
KW - Macrophages
KW - Male
KW - Monocytes
KW - Muscle, Skeletal
KW - Peptide Fragments
KW - Procollagen
KW - Tenascin
U2 - 10.1016/j.matbio.2004.05.009
DO - 10.1016/j.matbio.2004.05.009
M3 - Journal article
C2 - 15296940
VL - 23
SP - 259
EP - 264
JO - Matrix Biology
JF - Matrix Biology
SN - 0945-053X
IS - 4
ER -
ID: 38366948