Combined inhibition of nitric oxide and prostaglandins reduces human skeletal muscle blood flow during exercise
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Combined inhibition of nitric oxide and prostaglandins reduces human skeletal muscle blood flow during exercise. / Boushel, Robert Christopher; Langberg, Henning; Gemmer, Carsten; Olesen, Jens Raabjerg; Crameri, Regina; Scheede, Celena; Sander, Michael; Kjaer, Michael.
I: Journal of Physiology, Bind 543, Nr. Pt 2, 01.09.2002, s. 691-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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T1 - Combined inhibition of nitric oxide and prostaglandins reduces human skeletal muscle blood flow during exercise
AU - Boushel, Robert Christopher
AU - Langberg, Henning
AU - Gemmer, Carsten
AU - Olesen, Jens Raabjerg
AU - Crameri, Regina
AU - Scheede, Celena
AU - Sander, Michael
AU - Kjaer, Michael
PY - 2002/9/1
Y1 - 2002/9/1
N2 - The vascular endothelium is an important mediator of tissue vasodilatation, yet the role of the specific substances, nitric oxide (NO) and prostaglandins (PG), in mediating the large increases in muscle perfusion during exercise in humans is unclear. Quadriceps microvascular blood flow was quantified by near infrared spectroscopy and indocyanine green in six healthy humans during dynamic knee extension exercise with and without combined pharmacological inhibition of NO synthase (NOS) and PG by L-NAME and indomethacin, respectively. Microdialysis was applied to determine interstitial release of PG. Compared to control, combined blockade resulted in a 5- to 10-fold lower muscle interstitial PG level. During control incremental knee extension exercise, mean blood flow in the quadriceps muscles rose from 10 +/- 0.8 ml (100 ml tissue)(-1) min(-1) at rest to 124 +/- 19, 245 +/- 24, 329 +/- 24 and 312 +/- 25 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively. During inhibition of NOS and PG, blood flow was reduced to 8 +/- 0.5 ml (100 ml tissue)(-1) min(-1) at rest, and 100 +/- 13, 163 +/- 21, 217 +/- 23 and 256 +/- 28 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively (P <0.05 vs. control). In conclusion, combined inhibition of NOS and PG reduced muscle blood flow during dynamic exercise in humans. These findings demonstrate an important synergistic role of NO and PG for skeletal muscle vasodilatation and hyperaemia during muscular contraction.
AB - The vascular endothelium is an important mediator of tissue vasodilatation, yet the role of the specific substances, nitric oxide (NO) and prostaglandins (PG), in mediating the large increases in muscle perfusion during exercise in humans is unclear. Quadriceps microvascular blood flow was quantified by near infrared spectroscopy and indocyanine green in six healthy humans during dynamic knee extension exercise with and without combined pharmacological inhibition of NO synthase (NOS) and PG by L-NAME and indomethacin, respectively. Microdialysis was applied to determine interstitial release of PG. Compared to control, combined blockade resulted in a 5- to 10-fold lower muscle interstitial PG level. During control incremental knee extension exercise, mean blood flow in the quadriceps muscles rose from 10 +/- 0.8 ml (100 ml tissue)(-1) min(-1) at rest to 124 +/- 19, 245 +/- 24, 329 +/- 24 and 312 +/- 25 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively. During inhibition of NOS and PG, blood flow was reduced to 8 +/- 0.5 ml (100 ml tissue)(-1) min(-1) at rest, and 100 +/- 13, 163 +/- 21, 217 +/- 23 and 256 +/- 28 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively (P <0.05 vs. control). In conclusion, combined inhibition of NOS and PG reduced muscle blood flow during dynamic exercise in humans. These findings demonstrate an important synergistic role of NO and PG for skeletal muscle vasodilatation and hyperaemia during muscular contraction.
KW - Coloring Agents
KW - Cyclooxygenase Inhibitors
KW - Drug Synergism
KW - Endothelium, Vascular
KW - Enzyme Inhibitors
KW - Exercise
KW - Humans
KW - Indocyanine Green
KW - Indomethacin
KW - Microcirculation
KW - Microdialysis
KW - Muscle, Skeletal
KW - NG-Nitroarginine Methyl Ester
KW - Nitric Oxide
KW - Nitric Oxide Synthase
KW - Prostaglandins
KW - Spectroscopy, Near-Infrared
KW - Vasodilation
M3 - Journal article
C2 - 12205200
VL - 543
SP - 691
EP - 698
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - Pt 2
ER -
ID: 33850648