Can E. coli fly? The role of flies as transmitters of Escherichia coli to food in an urban slum in Bangladesh
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Can E. coli fly? The role of flies as transmitters of Escherichia coli to food in an urban slum in Bangladesh. / Lindeberg, Yrja Lisa; Egedal, Karen; Hossain, Zenat Zebin; Phelps, Matthew; Tulsiani, Suhella; Farhana, Israt; Begum, Anowara; Jensen, Peter Kjær Mackie.
I: Tropical Medicine & International Health, Bind 23, Nr. 1, 2018, s. 2-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Can E. coli fly?
T2 - The role of flies as transmitters of Escherichia coli to food in an urban slum in Bangladesh
AU - Lindeberg, Yrja Lisa
AU - Egedal, Karen
AU - Hossain, Zenat Zebin
AU - Phelps, Matthew
AU - Tulsiani, Suhella
AU - Farhana, Israt
AU - Begum, Anowara
AU - Jensen, Peter Kjær Mackie
N1 - This article is protected by copyright. All rights reserved.
PY - 2018
Y1 - 2018
N2 - OBJECTIVE: To investigate the transmission of fecal bacteria by flies to food under natural settings.METHODS: Over a period of two months paired (exposed and non-exposed) containers with cooked rice were placed on the ground in kitchen areas in an urban slum area in Dhaka, Bangladesh, and the numbers of flies landing on the exposed rice were counted. Following exposure, the surface of the rice was microbiologically and molecularly analysed for the presence of E. coli and genes of diarrheagenic E. coli and Shigella strains.RESULTS: Rice was at greater risk (p <0·001) of being contaminated with E. coli if flies landed on the rice than if no flies landed on the rice (odds ratio 5·4 (p < 0·001, 95% CI: 2·5 to 11·7). Mean contamination in exposed rice samples (n=60) was 3·1 x 103 CFU/g (95% CI: 2·2 x 103 to 4·0 x 103). Furthermore, for approximately half of the observed fly-landings, the average CFU per fly-landing was > 0·6 x 103 CFU. Genes of diarrheagenic E. coli and Shigella species were detected in 39 of 60 (65%) of exposed rice samples. Two fly species were identified; the common housefly (Musca domestica) and the oriental latrine fly (Chrysomya megacephala).CONCLUSION: Flies may transmit large quantities of E. coli to food under field settings. The findings highlight the importance of implementing control measures to minimize exposure of food to flies to ensure food safety. Fly control measures should be considered for the prevention of diarrheal diseases caused by E. coli. This article is protected by copyright. All rights reserved.
AB - OBJECTIVE: To investigate the transmission of fecal bacteria by flies to food under natural settings.METHODS: Over a period of two months paired (exposed and non-exposed) containers with cooked rice were placed on the ground in kitchen areas in an urban slum area in Dhaka, Bangladesh, and the numbers of flies landing on the exposed rice were counted. Following exposure, the surface of the rice was microbiologically and molecularly analysed for the presence of E. coli and genes of diarrheagenic E. coli and Shigella strains.RESULTS: Rice was at greater risk (p <0·001) of being contaminated with E. coli if flies landed on the rice than if no flies landed on the rice (odds ratio 5·4 (p < 0·001, 95% CI: 2·5 to 11·7). Mean contamination in exposed rice samples (n=60) was 3·1 x 103 CFU/g (95% CI: 2·2 x 103 to 4·0 x 103). Furthermore, for approximately half of the observed fly-landings, the average CFU per fly-landing was > 0·6 x 103 CFU. Genes of diarrheagenic E. coli and Shigella species were detected in 39 of 60 (65%) of exposed rice samples. Two fly species were identified; the common housefly (Musca domestica) and the oriental latrine fly (Chrysomya megacephala).CONCLUSION: Flies may transmit large quantities of E. coli to food under field settings. The findings highlight the importance of implementing control measures to minimize exposure of food to flies to ensure food safety. Fly control measures should be considered for the prevention of diarrheal diseases caused by E. coli. This article is protected by copyright. All rights reserved.
KW - Journal Article
U2 - 10.1111/tmi.13003
DO - 10.1111/tmi.13003
M3 - Journal article
C2 - 29121443
VL - 23
SP - 2
EP - 9
JO - Tropical Medicine & International Health
JF - Tropical Medicine & International Health
SN - 1360-2276
IS - 1
ER -
ID: 185622401